芒萁多糖提取及抗菌活性初步研究

采用4种方法提取芒萁多糖，其中综合法提取率为5．2％；可溶性多糖经DEAE-纤维素52柱层析进一步纯化，得到1种中性多糖和1种酸性多糖，各占78％和22％。利用纸片法进行抑菌实验结果表明，芒萁多糖显示了不同程度抑制细菌和真菌的活性。

Extraction of Dicranopteris dichotoma Polysaccharides and Its Antibiotic Activities

Dicranopteris dichotoma polysaccharide was extracted by four methods ,among those that the extracting rate of synthesis method was the highest ,reaching5.2%. The crude polysaccharide was purified by DEAE-52 and the neutral polysaccharide was eluted by distilled water and the polyanion polysaccharide was gradually eluted by 0.8mol/L NaCl. Their contents were 78% and 22%, respectively. The antibiotic activities of Dicranopteris dichotoma were tested with the agar disc diffusion method. The result showed that the polysaccharide had various amount of antibiotic activities against bacteria and fungi.