(1) Canola Research Station, Pioneer Hi-Bred Production Limited, 1211, Mississauga Road, RR #4, L7G 4S7 Georgetown, Ontario, Canada;(2) Plant Research Centre, Agriculture Canada, K1A OC6 Ottawa, Ontario, Canada
Abstract:
Approximately 2,000 embryogenic uninuclear microspores of rapeseed (Brassica napus) cv. Topas were intranuclearly injected with a chimaeric -glucuronidase (Escherichia coli Uid A) gene. Stable integration had not occurred among 55 plants that were regenerated. Coinjection of the dye Lucifer Yellow and detection of injected DNA by the polymerase chain reaction revealed high frequencies of transfer. However, the amount of DNA injected was less than 20 copies, which may have been insufficient for stable transformation of microspores.Abbreviations PCR polymerase chain reaction - GUS -glucuronidase