| 意蜂工蜂、雄蜂和蜂王的LDH和EST同工酶比较 |
| |
| 引用本文： | 邵邻相,杜震宇.意蜂工蜂、雄蜂和蜂王的LDH和EST同工酶比较[J].四川动物,2003,22(2):59-61. |
| |
| 作者姓名： | 邵邻相 杜震宇 |
| |
| 作者单位： | 浙江师范大学生命与环境科学学院,金华 321004 |
| |
| 摘 要： | 用聚丙烯酰胺凝胶电泳的方法，对意蜂工蜂、雄蜂和蜂王以及两种不同大小幼虫之间的乳酸脱氢酶(LDH)和酯酶(EST)同工酶分别作了比较。结果表明，工蜂、雄蜂和蜂王之间LDH同工酶数目无差异，但活性上存在差异；而酯酶同工酶在幼虫阶段与成虫阶段及工蜂、雄蜂和蜂王之间则有着不同的同工酶谱型。说明意蜂同工酶在种内的表达受到发育程度、进食质量、分化方向等因素的影响。
|
| 关 键 词： | 意蜂 工蜂 雄蜂 蜂王 LDH EST 同工酶 酯酶 乳酸脱氢酶 进化 分类 |
| 文章编号： | 1000-7083(2003)02-0059-03 |
Study on LDH and EST isoenzymes of queen bee, spado and drone of Italian honeybee |
| |
| SHAO Lin-xiang,DU Zhen-yu.Study on LDH and EST isoenzymes of queen bee, spado and drone of Italian honeybee[J].Sichuan Journal of Zoology,2003,22(2):59-61. |
| |
| Authors: | SHAO Lin-xiang DU Zhen-yu |
| |
| Abstract: | The paper deals with the lactate dehydrogenase (LDH) and esterase (EST) isoenzymes of queen bee, spado, drone and larvae with two different sizes of Italian honeybee (Apis mellifera) by using polyacrylamide gel electrophoresis and optical density scanning. The results showed that among queen bee, spado and drone, the number of LDH isoenzyme band was same but the activity of LDH was different. There existed obvious differences of EST isoenzyme between larva phase and adult phase and different isoenzyme zymogram in each intraspecific clade. It has been suggested by the authors that the degree of development, the direction of differentiation and the quality of food may influence the intraspecific expression of isoenayme. |
| |
| Keywords: | Italian honeybee lactate dehydrogenase (LDH) esterase (EST) isoenzymes |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
