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H19 non-coding RNA in urine cells detects urothelial carcinoma: a pilot study |
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| Authors: | Ilan Gielchinsky Michal Gilon Rasha Abu-lail Imad Matouk Avraham Hochberg Ofer N. Gofrit |
| Affiliation: | 1. Department of Urology, Hadassah – Hebrew University Medical Centre, Jerusalem, Israel;2. Department of Biological Chemistry, Institute of Life Sciences, the Hebrew University, Jerusalem, Israel |
| Abstract: | Context: Urothelial carcinoma (UC) is common and highly recurrent. Diagnosis and follow-up involve invasive cystoscopies. Objective: To evaluate H19 RNA in urine cells as diagnostic tool for UC. Materials and methods: RT-PCR analysis of urine samples from healthy volunteers and UC patients. Results: H19 RNA was unequivocally detected in the urine of 90.5% of patients and 25.9% of controls. H19 copies were three orders of magnitude higher in patients. Receiver operating characteristic analysis showed an area under the curve of 0.933. Conclusions: This pilot study shows that urinary cell H19 is a highly sensitive test for UC and pending verification could transform patient management. |
| Keywords: | Non-coding RNA diagnostic biomarkers urine biomarkers bladder cancer |