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CA9 as a biomarker in preoperative biopsy of small solid renal masses for diagnosis of clear cell renal cell carcinoma |
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| Authors: | Guorong Li Gang Feng Michel Péoc’h Michèle Cottier Nicolas Mottet |
| Institution: | 1. Department of Urology, CHU de Saint-Etienne, Saint-Etienne, France;2. Clinical Genetics Laboratory, Yijishan Hospital of Wannan Medical College, Wuhu, China;3. Laboratory of Pathology, CHU Saint-Etienne, Saint-Etienne, France;4. Inserm U1059, F-42055, Saint-Etienne, France;5. Laboratory of Cytopathology, CHU Saint-Etienne, Saint-Etienne, France |
| Abstract: | Purpose: The purpose of our study was to test the utility of CA9 expression in preoperative biopsy samples to identify the ccRCC. Materials and methods: A total of 55 patients with a small solid renal mass (≤4?cm) entered into this study. The immunohistochemical staining (51 samples) and RT-PCR (33 samples) were performed to detect CA9 expression. Results: For immunohistochemistry detection, CA9 was positive in 31/34 of biopsy samples of ccRCCs. CA9 was also positive in five suspected diagnosis of ccRCC. For RT-PCR detection, CA9 was positive in 25/25 biopsy samples of ccRCCs. The diagnostic accuracy of CA9 expression for ccRCC was 100%. RT-PCR was performed in four biopsies where immunohistochemistry could not be performed because of limited tissue materials or necrosis. Two ccRCCs with a negative staining by immunohistochemistry were CA9 positive by RT-PCR. Conclusions: CA9 may be potentially useful biomarker in help making a diagnosis of ccRCC in the biopsy of renal mass. RT-PCR might be a preferred method to immunohistochemistry for the detection of CA9 in renal biopsy samples. |
| Keywords: | Renal tumours biopsy CA9 immunohistochemistry RT-PCR |