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The relationship of single-strand breaks in DNA to breast cancer risk and to tissue concentrations of oestrogens |
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| Authors: | Mathavi Sahadevan Oukseub Lee Miguel Muzzio Belinda Phan Lisa Jacobs Nagi Khouri |
| Institution: | 1. Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA;2. Analytical Chemistry Division, IIT Research Institute, Chicago, IL, USA;3. Johns Hopkins School of Medicine, Breast Cancer Program, Kimmel Cancer Center, Baltimore, MD, USA |
| Abstract: | Context: Clinical study of breast cancer patients in Chicago, IL, USA. Objective: Ascertain the utility of measurements of single-strand breaks (SSB) in DNA for assessment of breast cancer risk. Methods: Fine-needle aspirates of the breast, SSB by nick translation, percent breast density (PBD), Gail model risk, cumulative methylation index (CMI), enzymes of DNA repair and tissue antioxidants. Results: DNA repair enzymes and 4-hydroxyestradiol were negatively associated with SSB; CMI and PBD were positively associated. Conclusions: Quantitative measurement of SSBs by this procedure indicates the relative number of SSBs and is related to promoter methylation, antioxidant availability and percent breast density. |
| Keywords: | Breast cancer single-strand breaks in DNA breast cancer risk tissue oestrogens DNA methylation |