A rapid, sensitive mass spectrometric method for investigating microscale chemical reactions of surface adsorbed peptides and proteins |
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| Authors: | B T Chait F H Field |
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| Affiliation: | 1. Lancaster Environment Centre, Lancaster University, Lancaster, United Kingdom;2. Smithsonian Tropical Research Institute, Panama, Republic of Panama;3. Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand;4. Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom;5. Department of Biology, Centre of Excellence PLECO (Plant and Ecosystems), University of Antwerp, Wilrijk, Belgium;6. INRA, UMR Ecology of Guiana Forests, AgroParisTech, Cirad, CNRS, Université des Antilles, Université de Guyane, Kourou, French Guiana;7. Integrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, CA, United States;8. Teagasc, Johnstown Castle, Wexford, Ireland |
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| Abstract: | A new mass spectrometric method for measuring the products of reactions of surface adsorbed peptides and proteins is described. The technique is rapid, convenient, and sensitive and provides detailed information concerning the molecular weights of the reaction products and the rate and extent of reaction. The properties of the technique are illustrated by an investigation of cleavage reactions of the disulfide bonds in bovine insulin, cyclic somatostatin, and conotoxin G1 utilizing the reducing agent dithiothreitol. |
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