Identification and characterization of a human cell line with dendritic cell features |
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Authors: | M Santamaria I Molina E Muñoz A Lopez M Toro J Peña |
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Institution: | 1. Department of Biochemistry Unit of Immunology, University of Cordoba Menendez Pidal s/n, E-14004, Cordoba, Spain 2. Department of Pathology, TUFTS University School of Medicine, Boston, USA 3. Pathology, School of Medicine, Reina Sofia Hospital, University of Cordoba Menendez Pidal s/n, E-14004, Cordoba, Spain
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Abstract: | CORP-4 is a cell line obtained in our laboratory from an explanted human bladder carcinoma. This cell line shows certain dendritic
cell features such as adherence to the culture plate surface, a doubling time of 24 h and an enzymatic profile typical of
cells involved in antigen presentation (non-specific esterases, lysozyme and α-1-antitrypsin). Its phenotypic analysis revealed
CD 15 and Fc receptor expression, S-100 surface protein and the presence of positive reactivity to different lectins such
as Concanavalin A (Con A) and Peanut agglutinin (PNA). CORP-4 was found to be a non-phagocytic cell line after it was assayed
with latex, and FcR- and C3bR-mediated phagocytosis. Furthermore, CORP-4 produced interleukin-1 (IL-1) as determined by thymocyte
proliferation assays and also fixes immune complexes in a non-complement dependent fashion. HLA class I and class II antigens
were inducible by both 5 azacytidine and gamma interferon. |
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Keywords: | Human dendritic line |
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