Biochemical studies on histones of the central nervous system. I. Characterization and partial identification of the label of histones from rat brain following intraventricular administration of 1-[14C]-acetate

Rat brain histones were acetylated in vivo by intraventricular injection of 14C]-acetate. More than 90% of the label is the result of a true acetylation. Enzymatic proteolysis of the labelled histone fraction and subsequent chromatographic investigation of the digestion products showed about 60% of the recovered radioactive material to be epsilon-acetyl lysine, whereas 22% of the radioactivity was found in an unidentified spot.