Identification of oxidation product of arbutin in mushroom tyrosinase assay system |
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| Authors: | Nihei Ken ichi Kubo Isao |
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| Affiliation: | Department of Environmental Science, Policy and Management, University of California, CA 94720-3112, Berkeley, USA. |
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| Abstract: | In order to elucidate whitening mechanisms of arbutin (hydroquinone-O-beta-D-glucopyranoside), its effects on mushroom tyrosinase were analyzed by spectrophotometric, polarographic, and HPLC experiments. It was found that as soon as catalytic amounts of L-DOPA become available as a cofactor, arbutin acts as a monophenol substrate. A significant enzymatic product was identified as 3,4-dihydroxyphenyl-O-beta-D-glucopyranoside by NMR and MS experiments. |
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