B型烟粉虱在6个烟草品种上的取食行为

【背景】不同烟草品种会影响B型烟粉虱的取食选择性,但具体的机制尚不明确。刺吸电波图（EPG）技术是研究刺吸式昆虫对寄主植物取食选择性的重要手段,通过烟粉虱取食产生的电信号,可以分析其对不同烟草品种的取食差异。【方法】应用EPG技术对B型烟粉虱雌成虫在6个烟草品种（翠碧1号、K326、闽烟7号、云烟85、云烟87、红花大金元）上的取食行为进行比较研究。【结果】6个烟草品种上有效记录的雌成虫数均为18头,在翠碧1号、K326、闽烟7号、云烟87、云烟85和红花大金元叶片上能够到达韧皮部的个体数分别为15、15、12、13、15和15头,能够持续吸食的个体数分别为13、14、8、12、14和15头。烟粉虱雌成虫在6个烟草品种上的非刺探（np）波所占的比例差异显著,在红花大金元上所占的比例最小,显著低于其他5个品种。雌成虫在红花大金元和K326上E1波的总持续时间显著短于闽烟7号和云烟87。雌成虫在红花大金元上E2波的总持续时间显著长于其他5个品种,而在闽烟7号、云烟87和云烟85上显著短于其他3个品种。【结论与意义】在6个烟草品种中,B型烟粉虱雌成虫最喜食红花大金元,最不喜食闽烟7号和云烟87。因此,在福建省B型烟粉虱的发生区,选择烟草品种时可根据实际情况减少红花大金元的种植,增多闽烟7号和云烟87等抗B型烟粉虱品种的种植。

Feeding behaviors of Bemisia tabaci B-biotype on six tobacco varieties

[Background]The feeding choice of Bemisia tabaci B-biotype can be affected by different tobacco varieties,but the specific mechanism is unclear.Electrical penetration graph（EPG） technique is an important method that can study feeding choice of piercing-sucking insects on host plants,and it can analyze the feeding differences of B.tabaci on different tobacco varieties,through the electrical signals change generated by feeding of B.tabaci.[Method]Feeding behaviors of female adults of B.tabaci B-biotype on 6 tobacco varieties（cv.CB-1,K326,Minyan 7,Yunyan 87,Yunyan 85 and Honghuadajinyuan） were observed by EPG technique.[Result]The feeding behaviors of 18 female adults were effectively recorded,on each variety.15,15,12,13,15 and 15 individuals reached phloem of cv.s CB-1,K326,Minyan 7,Yunyan 87,Yunyan 85 and Honghuadajinyuan respectively,and among them,13,14,8,12,14 and 15 individuals could ingest plant sap continuously.There was significant different in the percentage of non-probing of female adults on different varieties.The percentage of non-probing on Honghuadajinyuan was the smallest.Total duration of salivation into sieve elements（waveform E1） on cv.s Honghuadajinyuan and K326 were significantly shorter than those on cv.s Minyan 7 and Yunyan 87;The total duration of phloem ingestion（waveform E2） on Honghuadajinyuan were significantly longer than others;The times of all E2 periods on cv.s Minyan 7,Yunyan 87 and Yunyan 85 were significantly shorter than those on other three varieties.[Conclusion and significance]Female adults of B.tabaci B-biotype preferred cv.Honghuadajinyuan,and least preferred cv.s Minyan 7 and Yunyan 87 among the examined tobacco cultivars.Therefore,in Fujian Province where B.tabaci B-biotype is present/invading,planting tobacco cv.Honghuadajinyuan would be advantageous,and B.tabaci B-biotype-resistant varieties such as cv.Minyan 7 and Yunyan 87 are more widely planted than currently.