| 小鼠生长过程中肝、肺、肾胶原蛋白与MMP-1含量变化 |
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| 引用本文： | 高建萍,张扬,邢芳毓,孔英俊,张贵锋. 小鼠生长过程中肝、肺、肾胶原蛋白与MMP-1含量变化[J]. 生物工程学报, 2021, 37(2): 646-654 |
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| 作者姓名： | 高建萍 张扬 邢芳毓 孔英俊 张贵锋 |
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| 作者单位： | 1 中国科学院 过程工程研究所 生化工程国家重点实验室，北京 100190;2 中国科学院大学 化学工程学院，北京 100049 |
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| 基金项目： | 国家重点研发计划 (Nos. 2018YFA0108200, 2018YFC1106400)，广州市科技计划项目 (No. 201803010086) 资助。 |
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| 摘 要： | 利用高效液相色谱法(High performance liquid chromatography,HPLC)和酶联免疫吸附实验(Enzyme-linked immunoassay,ELISA)对小鼠生长过程中肝、肺、肾胶原蛋白与基质金属蛋白酶-1(Matrix metalloproteinase-1,MMP-1)含量变...
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| 关 键 词： | 生长过程 肝脏 肺脏 肾脏 胶原蛋白含量 基质金属蛋白酶-1 |
| 收稿时间： | 2020-03-18 |
Changes of collagen and MMP-1 in liver, lung and kidney during growth of mice |
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| Jianping Gao,Yang Zhang,Fangyu Xing,Yingjun Kong,Guifeng Zhang. Changes of collagen and MMP-1 in liver, lung and kidney during growth of mice[J]. Chinese journal of biotechnology, 2021, 37(2): 646-654 |
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| Authors: | Jianping Gao Yang Zhang Fangyu Xing Yingjun Kong Guifeng Zhang |
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| Affiliation: | 1 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;2 School of Chemical and Engineering, University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The high performance liquid chromatography (HPLC) and enzyme-linked immunoassay (ELISA) were used to investigate the changes of collagen and matrix metalloproteinase-1 (MMP-1) in liver, lung and kidney during growth process of mice. The mice from 0 to 18 weeks were used as the research objects. The contents and proportions of hydroxyproline (Hyp), which were used to calculate the collagen contents, in liver, lung and kidney of different weeks were analyzed with HPLC. The contents and activity of MMP-1 in liver, lung and kidney of different weeks were analyzed with ELISA. The results showed that the collagen contents in liver, lung, and kidney were different (Lung(COL)>Kidney(COL)>Liver(COL)), and they all increased first and then decreased with weeks. The collagen contents in liver, lung, and kidney reached the highest level in the ninth (5.52 ng/mg), sixth (54.10 ng/mg) and ninth (19.20 ng/mg) week, respectively. Then it declined slowly from 9 to 18 weeks. The result of ELISA showed that the MMP-1 contents in liver, lung and kidney decreased first and then increased with weeks, and the trend of MMP-1 activity was opposite. It indicated that the increase of collagen contents in the tissues will inhibit the secretion of MMP-1. |
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| Keywords: | growth process liver lung kidney collagen content matrix metalloproteinase-1 |
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