Digital quantification using amplified single-molecule detection |
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Authors: | Jarvius Jonas Melin Jonas Göransson Jenny Stenberg Johan Fredriksson Simon Gonzalez-Rey Carlos Bertilsson Stefan Nilsson Mats |
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Affiliation: | Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, Sweden. |
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Abstract: | We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae. |
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