Genome-scale loss-of-function screening with a lentiviral RNAi library |
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Authors: | Root David E Hacohen Nir Hahn William C Lander Eric S Sabatini David M |
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Institution: | Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA. droot@broad.mit.edu |
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Abstract: | The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens. |
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