Filamentous fungi as production organisms for glycoproteins of bio-medical interest |
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| Authors: | Marleen Maras Irma van Die Roland Contreras Cees AMJJ van den Hondel |
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| Institution: | (1) Department of Molecular Biology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium;(2) Department of Medical Chemistry, Vrije Universiteit, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands;(3) Department of Molecular Genetics and Genetechnology, TNO Nutrition and Food Research Institute, P.O. Box 360, 3700 AJ Zeist, The Netherlands |
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| Abstract: | Filamentous fungi are commonly used in the fermentation industry for large scale production of glycoproteins. Several of these proteins can be produced in concentrations up to 20–40 g per litre. The production of heterologous glycoproteins is at least one or two orders of magnitude lower but research is in progress to increase the production levels. In the past years the structure of protein-linked carbohydrates of a number of fungal proteins has been elucidated, showing the presence of oligo-mannosidic and high-mannose chains, sometimes with typical fungal modifications. A start has been made to engineer the glycosylation pathway in filamentous fungi to obtain strains that show a more mammalian-like type of glycosylation. This mini review aims to cover the current knowledge of glycosylation in filamentous fungi, and to show the possibilities to produce glycoproteins with these organisms with a more mammalian-like type of glycosylation for research purposes or pharmaceutical applications |
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| Keywords: | filamentous fungi homologous and heterologous protein secretion N-glycosylation O-glycosylation phosphorylation protein-linked carbohydrates |
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