Diffusion of the mu opioid receptor at the surface of human neuroblastoma SH-SY5Y cells is restricted to permeable domains |
| |
Authors: | Saulière Aude Gaibelet Gérald Millot Claire Mazères Serge Lopez André Salomé Laurence |
| |
Institution: | Institut de Pharmacologie et Biologie Structurale, UMR CNRS 5089, 205, route de Narbonne, 31077 TOULOUSE cedex, France. |
| |
Abstract: | Previous single-molecule studies have shown a long-term diffusion superimposed to a short-term confinement of the human mu opioid (hMOP) receptors at the surface of heterologous cells. However, additional ensemble average measurements are required to reach a complete understanding of the undergoing process. Here, we analyse, by fluorescence recovery after photobleaching measurements, the lateral diffusion of fully functional T7-EGFP-hMOP receptors in neuroblastoma SH-SY5Y cells naturally expressing a low level of the wild-type receptor. Experiments carried out at variable observation radii demonstrate the restriction of the receptors diffusion to sub-micrometer sized domains. Furthermore, consistently with the long-term single-molecule data, the domains are found permeable. |
| |
Keywords: | hMOP human mu opioid GPCR G-protein-coupled receptor DAMGO d-Ala2 N-Me-Phe4Gly-ol5]enkephalin" target="_blank">[d-Ala2 N-Me-Phe4Gly-ol5]enkephalin CTAP d-Phe-Cys-Tyr-d-Trp-Arg-Thr-Pen-Thr-NH2" target="_blank">d-Phe-Cys-Tyr-d-Trp-Arg-Thr-Pen-Thr-NH2 DPN diprenorphine cAMP cyclic adenosin monophosphate |
本文献已被 ScienceDirect PubMed 等数据库收录! |