Laser microirradiation of the nucleolar organizer in cells of the rat kangaroo (Potorous tridactylis). Reduction of nucleolar number and production of micronucleoli |
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| Authors: | M W Berns A D Floyd K Adkisson W K Cheng L Moore G Hoover K Ustick S Burgott T Osial |
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| Affiliation: | 1. Department of Zoology, University of Michigan, Ann Arbor, Mich. 48104, USA;2. Department of Anatomy, University of Michigan, Ann Arbor, Mich. 48104, USA;1. Molecular Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA;1. Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA, 92093, USA;2. Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA, 92093, USA |
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| Abstract: | Cells of the established cell lines of the rat kangaroo (Protorous tridactylis), were laser microirradiated in order to study their nucleolar organizers. Microbeam irradiation of prophase and metaphase nucleolar organizer sites in both male (PTK1) and female (PTK2) cells resulted in a reduction in the number of normal nucleoli formed following mitosis. Irradiation of the one nucleolar organizer in the male cells, often resulted in the formation of numerous micronucleoli. Micronucleoli were also formed in small, extra nuclei, that were probably formed as a result of microirradiation of the centromere region of autosomes. Preliminary cytochemical analysis of the micronucleoli is presented and the possible mechanism of the formation is discussed. |
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