Rapid analysis of amplified double-stranded DNA by capillary electrophoresis with laser-induced fluorescence detection |
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Authors: | Ming-Sun Liu Fu-Tai Albert Chen |
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Institution: | (1) Beckman Coulter Inc., 4300 N. Harbov Blvd., 92834 Fullerton, CA |
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Abstract: | DNA amplification technology has been applied to clinical diagnosis of infectious disease, genetic disorder, and cancer. After
in vitro amplification of a particular DNA region, the methods of analysis for these amplified samples play a pivotal role
in clinical diagnosis. Conventional gel electrophoresis has been routinely used in the lab for checking DNA. The whole procedure
is time consuming and requires more than 1 ng of DNA for detection. To achieve greater performance in DNA diagnosis, we demonstrated
capillary electrophoresis with laser induced fluorescence detection for analysis of amplified DNA. The analysis of DNA could
be completed within 3 min and the data is directly entered into the computer. Considering the automatic and rapid process,
we believe that this method could be routinely utilized for the clinical diagnosis of amplified DNA products. |
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Keywords: | Capillary electrophoresis double-stranded DNA analysis DNA amplification laser-induced fluorescence detection |
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