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Biochemical genetic relationship of Thailand and Hawaii isolates of Parastrongylus cantonensis (Nematoda: Angiostrongylidae)
Authors:Praphathip Eamsobhana  Naowarat Dechkum  H.S. Yong
Affiliation:1. Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand;2. Department of Parasitology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand;3. Institute of Biological Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia
Abstract:The genetic relationship of the Thailand and Hawaii isolates (strains) of the rat lungworm Parastrongylus (=Angiostrongylus) cantonensis was investigated by gene–enzyme systems using vertical polyacrylamide slab gel electrophoresis. Six gene–enzyme systems were successfully determined, with each being represented by two presumptive loci. Glucose phosphate dehydrogenase, glucose phosphate isomerase, lactate dehydrogenase, malate dehydrogenase and malic enzyme were monomorphic at both loci, and the respective bands exhibited similar mobility in both isolates implying absence of genetic variation.
Keywords:Parastrongylus cantonensis   Angiostrongylus   Nematode   Gene&ndash  enzyme system   Geographical isolate   Angiostrongylidae
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