Synthesis of alpha subunit of human chorionic gonadotrophin by presumptive HeLa cells |
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| Authors: | Janice Y. Chou Bruce D. Weintraub Saul W. Rosen Jacqueline Whangpeng Howard D. Sussman Joan R. Haughom J. C. Robinson |
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| Affiliation: | (1) Laboratory of Biomedical Sciences, National Institute of Child Health and Human Development, National Institutes of Health, 20014 Bethesda, Md.;(2) Clinical Endocrinology Branch, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, 20014 Bethesda, Md.;(3) Cytogenic Oncology Section, National Cancer Institute, National Institutes of Health, 20014 Bethesda, Md.;(4) Laboratory of Experimental Oncology, Department of Pathology, Stanford University Medical School, 94305 Stanford, Calif.;(5) Flow Laboratories, 20852 Rockville, Md. |
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| Abstract: | Summary Several cell lines, originally thought to be derived from a human placenta at term but possibly HeLa-contaminated, have been studied. These cells secrete a protein indistinguishable immunochemically from the alpha subunit of chorionic gonadotropin but not the beta subunit of chorionic gonadotropin or placental lactogen. Complete chorionic gonadotropin was detected but amounted to less than 1% of the level of the alpha subunit. The cells also produce an alkaline phosphatase similar to placental alkaline phosphatase in immunochemical, gel-electrophoretic, and heat-denaturation properties. They induce tumor growth when inoculated into nude mice. These cells are aneuploid and have a model chromosome number of 66. The common HeLa karyologic markers, designated 1, 2, and 3, and A-type glucose-6-phosphate dehydrogenase are present in these cells. HeLa cells have not previously been shown to secrete theα subunit of hCG. |
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| Keywords: | placental proteins human cells gonadotrophin |
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