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Comparison of biochemical and molecular genetic approaches for identification of environmental strains |
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| Authors: | S O Korshunov A V Gorovtsov T G Faleeva S N Gorbov I V Kornienko |
| Institution: | 1. Southern Federal University, ul. Bolshaya Sadovaya 105/42, Rostov-on-Don, 344006, Russia 2. Rostov State Medical University, Nakhichevanskiy per. 29, Rostov-on-Don, 344022, Russia 3. 16 State Center for Forensic Medical and Criminal Expertise, Southern Military District, the Ministry of Defense of the Russian Federation, ul. Lermontovskaya 60, Rostov-on-Don, 344010, Russia |
| Abstract: | In order to confirm the taxonomic position of environmental strains determined based on their biochemical, cultural, and morphological characteristics, molecular genetic identification was carried out. A number of problems in identification of microorganisms were shown to be associated with contamination of the cultures in the course of isolation. Advantages of a comprehensive approach—combining 16S rRNA gene sequencing with a set of biochemical, cultural, and morphological parameters—for identification of microorganisms isolated from environmental objects and clinical samples are discussed. |
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