胭脂鱼眼早期形态发生研究

采用组织学方法观察了胭脂鱼(Myxocyprinus asiaticus) 眼的发生过程, 结果显示: 胭脂鱼眼的发育经历了眼原基形成期、眼囊形成期、视杯形成期、晶体板形成期、晶体囊形成期、角膜原基形成期、角膜上皮形成期、视网膜细胞增殖期、晶状体成熟期、眼色素形成期以及眼成型期等11个时期。视网膜发育最早, 起始于眼原基的形成, 直至眼成型期分化完成, 形成了厚度不一的8层细胞, 由内向外依次为神经纤维层、神经细胞层、内网层、内核层、外网层、外核层、视杆视锥层和色素上皮层, 且发育历时最长, 约264h。晶状体的发育在视网膜之后, 始于晶体板的形成, 于出膜前期成熟, 发育历时最短, 约74h。角膜发育最晚, 始于角膜原基的形成, 出膜1 d分化为透明的成熟角膜, 发育历时约96h。出膜4 d仔鱼眼色素沉积明显, 视网膜各层分化明显, 晶状体内部完全纤维化, 眼的形态结构基本发育完全。

EARLY EYE MORPHOGENESIS IN THE CHINESE SUCKER (MYXOCYPRINUS ASIATICUS)

The present study investigated the eye morphogenesis of Myxocyprinus asiaticus by histological methods. The results showed that the development of eye was involved 11 stages, including optic rudiment, optic vesicle, optic cup, lens placode, lens capsule, cornea anlage, cornea, retina cell multiplication, lens maturity, ommochrome formation, and eye formed in order. The retinal was firstly developed and then formed the different thickness of 8 layers of cells, including the inside-out, followed optic fiber layer, ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, rod-cone cell and the pigment epithelium, with the longest period (about 264h) from the stages of optic rudiment formation, and lasted until completed eye formation. The development of lens followed after the formation of retinal took a shortest time (74h), which began in the formation of lens placode and matured before hatching. Corneal developed lastly, and its development took about 96h from the formation of cornea anlage to the maturation, and transparent cornea developed at 1 day after hatching. Pigment deposition was significantly observed at the 4-day-old fish larvae and the layers of retina distinctly differentiated. Lens developed with fibrosis and then eye development completed.