用混合氮源在室外培养产油链带藻的可行性研究

为探索两株链带藻（Desmodesmus sp.T28-1和Desmodesmus sp.NMX451）在室外培养的最优氮源，首先在室内就不同氮源（尿素、硝酸钠、碳酸铵以及尿素和硝酸钠混合氮源）下微藻的生长和油脂积累做了研究，筛选出最优的混合氮源在室外进行了培养的可行性研究。室内研究结果表明两株链带藻在尿素下培养油脂含量最低，在铵氮下培养生物量最低。且NMX451在混合态氮下的油脂产率显著性的高于其他氮源下的油脂产率。对两株链带藻在混合氮源下的脂肪酸组分做进一步分析，结果表明油脂组分适合生物柴油生产要求，估算的生物柴油品质达到国际和国内生产标准。将两株链带藻置于室外140 L柱式反应器中用混合氮源进一步扩大培养，结果表明NMX451比T28-1的油脂含量和油脂产率高，生产成本更低，且脂肪酸组分更适宜生物柴油生产。研究表明用混合氮源在室外培养微藻是非常可行的培养方法，也说明NMX451比T28-1在生物柴油生产方面具有更好的潜力。

THE FEASIBILITY OF CULTIVATION OIL-RICHDESMODESMUS UNDER MIXED NITROGEN SOURCE

To explore the optimum nitrogen source for outdoor cultivation ofDesmodesmus sp. T28-1 andDesmo-desmus sp. NMX451, this study investigated effects of different nitrogen sources (urea, nitrate, ammonium and com-bined urea and nitrate) on the growth and lipid production and evaluated the best mixed nitrogen source for outdoor cul-tivation. The results of indoor cultivation showed that both T28-1and NMX451 had the lowest lipid content under urea and the lowest biomass productivity under ammonium. NMX451 obtained significantly higher lipid productivity under the combination of urea and nitrate than any other nitrogen source. Both T28-1 and NMX451 obtained high biodiesel quality under mixed nitrogen source, which met the standards of America, Europe and China. Results of cultivating the two microalgae with mixed nitrogen source in an outdoor 140 L photobioreactor showed that NMX451 had higher li-pid content and lipid productivity and even better biodiesel quality than cultivation indoor. These results suggest that mixed nitrogen source can be used to produce microalgae-based biodiesel production outdoors, and NMX451 had bet-ter biofuel production under this condition than T28-1.