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| 构建多顺反子表达载体的有效工具——FMDV 2A | |
| 引用本文： | 刘必胜,刘新垣,钱程. 构建多顺反子表达载体的有效工具——FMDV 2A[J]. 生物工程学报, 2007, 23(5): 765-769 |
| 作者姓名： | 刘必胜 刘新垣 钱程 |
| 作者单位： | 1. 浙江理工大学新元医学与生物技术研究所,杭州,310018 2. 浙江理工大学新元医学与生物技术研究所,杭州,310018;中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海,200031 3. 浙江理工大学新元医学与生物技术研究所,杭州,310018;Division of Gene Therapy and Hepatology, Medical School, University of Narrava, 31008, Spain |
| 基金项目： | 国家重点基础研究发展计划(973计划);浙江省科技厅科技计划 |
| 摘 要： | 近年来肿瘤多基因治疗倍受关注,然而目前缺少一种有效的构建多顺子的工具。传统的构建多顺反子的工具,如IRES等,由于存在结构大,上下游基因表达差异显著等缺陷,严重限制了其应用。FMDV2A,因其具有结构小、剪切效率高等优点为多基因治疗带来了新的希望。主要介绍了FMDV2A的特性、"剪切"活力及其在构建多顺反子载体中的应用。 |
| 关 键 词： | 多顺反子 基因治疗 肿瘤 |
| 文章编号： | 1000-3061（2007）05-0765-05 |
| 修稿时间： | 2007-01-15 |
An Efficient Tool for the Construction of Multiple-cistronic Vectors: FMDV 2A |
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| LIU Bi-Sheng,LIU Xin-Yuan and QIAN Cheng. An Efficient Tool for the Construction of Multiple-cistronic Vectors: FMDV 2A[J]. Chinese journal of biotechnology, 2007, 23(5): 765-769 | |
| Authors: | LIU Bi-Sheng LIU Xin-Yuan QIAN Cheng |
| Affiliation: | 1 Xinyuan Institute of Medicine and Biotechnology;School of Life Sciences;Zhejiang Sci-Tech University;Hangzhou 310018;China2 Institute of Biochemistry and Cell Biology;Shanghai Institutes for Biological Sciences;Chinese Academy of Sciences;Shanghai 200031;China3 Division of Gene Therapy and Hepatology;Medical School;University of Narrava 31008;Spain |
| Abstract: | Recently,cancer therapy with mutiple genes has been attached with great attention.However,at present there is no efficient tool to construct multiple-cistrons.The large sizes and the imbalance in expression of most traditional tools,such as ribosome entry sites(IRESes),greatly block their wide employment in the construction of multiple cistronic gene therapy vectors.The self-cleaving peptide 2A from foot-and-mouth disease virus(FMDV)has a very small size,and more importantly,high cleavage activity in artifical bicistron,which bring new hope for mutiple genes therapy stategy.In this article,the characteristics and cleavage activities of FMDV 2A will be elucidated,and we further outline its applications in cancer gene therapy. |
| Keywords: | FMDV 2A |
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