A mutant of Mycoplasma mycoides subsp. mycoides lacking the H2O2-producing enzyme l-α-glycerophosphate oxidase |
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| Authors: | BJ Wadher CL Henderson RJ Miles H Varsani |
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| Institution: | Department of Microbiology, Gifu University School of Medicine, Japan. |
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| Abstract: | A universal rapid procedure to determine the DNA base composition (mol% guanine + cytosine) of Gram-positive bacteria is described. Cells of Gram-positive bacteria were lysed with achromo-peptidase and the mol% G + C of their DNAs were determined by using high performance liquid chromatography. One ml of a Gram-positive bacterial suspension which matched MacFarland No. 3 standard turbidity was sufficient to determine the mol% G + C within 3 h. |
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| Keywords: | Gram-positive bacterium Rapid procedure DNA base composition |
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