Identification and purification of human erythroid progenitor cells by monoclonal antibody to the transferrin receptor (TU 67) |
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| Authors: | F Herrmann J D Griffin K D Sabbath W Oster P Wernet R Mertelsmann |
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| Institution: | Abteilung für H?matologie, I. Medizinische Klinik, Johannes Gutenberg-Universit?t, Mainz, Federal Republic of Germany. |
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| Abstract: | Anti-TU 67 is a murine monoclonal antibody that recognizes the transferrin receptor. With respect to hematopoietic cells TU 67 is expressed by human multipotent colony-forming cells (CFU-Mix), erythroid progenitor cells (BFU-E and CFU-E) and a fraction of granulocyte/monocyte colony forming cells, but is not expressed by mature hematopoietic cells including erythrocytes, platelets, lymphocytes, and peripheral blood myeloid cells. The TU 67-positive fraction of normal bone marrow, separated by fluorescence-activated cell sorting (FACS) or immune rosettes, contained 87% of the erythroid progenitor cells. Erythroid progenitor cells were enriched up to 50-fold by using a combination of monoclonal antibodies to deplete mature hematopoietic cells, followed by positive selection of BFU-E and CFU-E by TU 67 antibody. |
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