Glucose suppression of deoxyribose interference in the thiobarbituric acid determination of sialic acid. |
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| Authors: | C H Smith T M Donohue R Depper |
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| Affiliation: | 1. Department of Pediatrics, Washington University School of Medicine, St. Louis Children''s Hospital, St. Louis, Missouri 63110 USA;2. Department of Pathology, Washington University School of Medicine, St. Louis Children''s Hospital, St. Louis, Missouri 63110 USA |
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| Abstract: | Glucose has been demonstrated to suppress the reaction of deoxyribose in the thiobarbituric acid determination of sialic acid. Suppression occurs at the periodate oxidation step in which glucose apparently competes with deoxyribose. This suppression is augmented by sodium chloride and trichloroacetic acid. With these three substances, deoxyribose reactivity can be completely eliminated at concentrations up to 75 μm with only a 25% decrease in sialic acid reactivity.Mixtures of deoxyribose and sialic acid and hydrolyzed extracts of rat organs gave reaction products with an absorption spectrum closely resembling that given by a sialic acid standard. Provided that the spectral characteristics of the colored product from an unknown sample are verified, sialic acid can be determined directly from absorbance at 550 nm without interference by deoxyribose. |
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