Effect of thidiazuron,light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves |
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| Authors: | Joseph A Fiola Mahmoud A Hassan Harry Jan Swartz Robert H Bors Ronnie McNicols |
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| Institution: | (1) UN/FRD Research Unit for Plant Growth and Development, Department of Botany, University of Natal, 3200 Pietermaritzburg, RSA;(2) Vegetable and Ornamental Plant Research Institute, Private Bag X293, 0001 Pretoria, RSA |
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| Abstract: | The relationship between genotype, tissue age and endogenous cytokinin levels on adventitious bud formation on Lachenalia leaf tissue were investigated. The genotypes studied, showed a variation in bud formation. The hybrid explants responded differently to factorial combinations of BA and NAA. The growth regulators could not substitute for the regeneration potential of the genotype. Tissue age had a pronounced effect on regeneration potential. Young tissue formed the largest number of buds. An interaction between tissue age and genotype was detected. Cytokinin levels in young leaf tissue were higher than in older tissue. In young tissue no relationship was observed between the cytokinin level and the number of buds formed. However, in older tissue it appears as if a relatively low endogenous cytokinin level enhanced bud formation.Abbreviations BA
benzyladenine
- NAA
naphthalene-1-acetic acid
- Z
zeatin
- ZR
ribosylzeatin |
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| Keywords: | adventitious bud formation cytokinins effect of age and genotype Lachenalia |
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