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Swelling of Etiolated Oat Protoplasts Induced by Phytochrome, cAMP and Gibberellic Acid: A Kinetic Study |
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| Authors: | Chung Chang Ho; Lim Sun-Uk; Song Pill-Soon; Berlin Jerry D; Goodin Joe R |
| Institution: | 1Department of Chemistry and Biochemistry, Texas Tech University Lubbock, TX 79409, U.S.A. 2Department of Agricultural Chemistry, Seoul National University Suwon, Korea 3Department of Chemistry and Section of Molecular Plant Biology, School of Biological Sciences, University of Nebraska Lincoln, NE 68588-0304, U.S.A. 4Department of Biological Sciences, Texas Tech University Lubbock, TX 79409, U.S.A. |
| Abstract: | The effects of phytochrome/light and other regulatory agentson the swelling of protoplasts from the primary leaves of etiolatedoat seedlings have been investigated. The protoplasts did notswell in darkness. Red (R) light immediately followed by far-red(FR) or FR light treatment alone for 4 h induced swelling slowly.In comparison, the protoplasts treated with R or FR-R swellmore rapidly and with a shorter lag period. The effect of redlight on the protoplast swelling was photoreversible by FR,suggesting the involvement of endogenous phytochrome. Exogenous gibberellic acid (GA3) or dibutyryl cAMP (DBcAMP)stimulated the effect of R irradiation on the protoplast swelling.As with R irradiation, these agents were sufficient to causethe swelling of protoplasts in the dark with a shorter lag periodthan those maintained in darkness or a 5 min FR. The combinationof a 5 min R irradiation and GA3 showed a synergistic effecton the enlargement of protoplast size. On the other hand, theincrease in protoplast size was proportional to the concentrationof DBcAMP with or without a 5 min R irradiation. (Received February 29, 1988; Accepted May 13, 1988) |
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