Characterization of acid phosphatase-1 null activity mutants of Drosophila melanogaster

A complementation analysis was performed on 15 Acph-1ⁿ alleles. Only three of these alleles proved to be nonleaky and to exhibit no evidence of complementation. These were then tested immunologically to determine their level of antigenically cross-reacting material (CRM). Their CRM levels were virtually zero, as compared to low, but positive, levels for two other homozygous Acph-1ⁿ mutants and considerably higher levels for heteroallelic combinations of some of the leaky alleles. Acid phosphatase-1 enzyme subunits, formed by dissociating native enzyme, have close to 100% CRM activity in tests with antibodies elicited by native enzyme.Submitted by John Bell in partial fulfillment of the requirements for a Doctor of Philosophy degree from Cornell University.