Ribonuclease A--a complete proton NMR fingerprint using TOCSY and NOESY experiments in water |
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Authors: | L Y Lian |
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Institution: | Biological NMR Centre, University of Leicester, United Kingdom. |
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Abstract: | In our attempts to obtain total sequential assignment of the 1H NMR spectrum of ribonuclease A, several published water suppression techniques were tested and assessed. The jump- and-return sequence and its echo hybrid were used with considerable success in both the TOCSY and NOESY experiments on 3mM ribonuclease A solutions. The NMR approach used here may be of general applicability for 1H NMR studies of proteins in water of concentrations under 5mM. |
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