DNA-binding proteins in xeroderma pigmentosum fibroblasts. |
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Authors: | A R Lehmann S Kirk-Bell |
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Institution: | MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton BN1 9QG, UK |
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Abstract: | DNA-binding proteins in human fibroblasts were examined by chromatography on DNA-cellulose columns. By successive chromatography on columns containing native, denatured, and UV-irradiated DNA-cellulose respectively the proteins binding to different types of DNA could be studied. Elution of the columns with sodium chloride followed by polyacrylamide gel electrophoresis allowed several DNA-binding proteins to be identified. All of the major DNA-binding proteins were present in strains of xeroderma pigmentosum cells respectively deficient in excision-repair and post-replication repair of ultraviolet-induced damage. |
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