徐州权台煤矿井下700 m水平微生物群落分布特征

煤矿井下与表生环境具有明显差异,研究其微生物群落分布及多样性对深入探讨井下水-岩-气-生作用机制具有重要意义.本研究在徐州权台煤矿井下700 m水平采集了8个沉积物样本,对样品进行了理化指标测试和Miseq高通量测序.结果表明,煤矿井下具有丰富的微生物多样性,8个样本共检测到35个细菌门类,其中变形菌门(Proteob...

Distribution characteristics of microbial communities at a depth of 700 m level of Quantai coal mine in Xuzhou

There are obvious differences between underground coal mine and surface environment.The studies of the distribution and diversity of microbial community in the underground coal mines are important for exploring the water-rock-gas-biological mechanism.In this study,eight coal deposit samples were collected from the level of-700 m in Quantai Coal Mine,Xuzhou,East China.The physical and chemical indices of the samples were tested and Miseq highthroughput sequencing was performed.A total of 35 bacteria phyla were detected in the eight samples,with Proteobacteria,Firmicutes,and Actinobacteria together accounting for more than 80%of the total abundance.Proteobacteria and Firmicutes were mainly distributed in rock lanes,while Actinobacteria were mainly distributed in coal lanes.The dominant bacterial genera of coal mines included Lactococcus,Pseudomonas,Mycobacterium,and Bacillus.In general,the abundance and diversity of bacterial communities in rock lanes were higher than those in coal lanes.pH was the key factor affecting the abundance of bacterial community in coal mine(R2=0.766,P<0.05)at the phylum level.pH was positively correlated with Actinobacteria,Firmicutes and Latescibacteria,while negatively correlated with other phyla.The concentrations of total organic carbon(TOC),total nitrogen(TN),total phosphorus(TP)and sulfate also affected the community abundance distribution.Therefore,the influence of underground microorganisms should be fully considered when examining coal mine pollution mechanism and pollutant migration.