| U2AF65蛋白表达水平影响基因UBQLN1的可变剪接 |
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| 引用本文： | 瞿秀华,刘萱,李晓明,李平,马清钧,曹诚.U2AF65蛋白表达水平影响基因UBQLN1的可变剪接[J].生物技术通讯,2010,21(4):470-473. |
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| 作者姓名： | 瞿秀华 刘萱 李晓明 李平 马清钧 曹诚 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100850 |
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| 摘 要： | 目的:研究U2AF65蛋白的表达水平对基因UBQLN1可变剪接的影响。方法:应用pSR-GFP/Neo载体构建2个U2AF65-siRNA干扰载体,转染293T细胞,通过Western印迹、QRT-PCR检测干扰效果,RT-PCR验证基因UBQLN1的可变剪接。结果:利用设计的U2AF65-siRNA能够干扰细胞中U2AF65的表达;RT-PCR结果显示U2AF65表达水平的下降促使UBQLN1第8外显子的跳跃增加。结论:U2AF65可以通过表达水平的变化参与调控基因UBQLN1的可变剪接。
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| 关 键 词： | U2AF65蛋白 RNA干扰 UBQLN1基因 可变剪接 |
Changes of U2AF65 Expression Level Affect Alternative Splicing of UBQLN1 Gene |
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| QU Xiu-Hua,LIU Xuan,LI Xiao-Ming,LI Ping,MA Qing-Jun,CAO Cheng.Changes of U2AF65 Expression Level Affect Alternative Splicing of UBQLN1 Gene[J].Letters in Biotechnology,2010,21(4):470-473. |
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| Authors: | QU Xiu-Hua LIU Xuan LI Xiao-Ming LI Ping MA Qing-Jun CAO Cheng |
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| Institution: | QU Xiu-Hua,LIU Xuan,LI Xiao-Ming,LI Ping,MA Qing-Jun,CAO Cheng Beijing Institute of Biotechnology,Beijing 100850,China |
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| Abstract: | Objective: To investigate the effect of U2AF65 expression level on alternative splicing of UBQLN1 gene. Methods: Two U2AF65-siRNA vectors were constructed by using the pSR-GFP / Neo vector, and then they were transfected into 293T cells. The effect of RNA interference was examined by methods of Western blotting and QRT-PCR, and the alternative splicing of UBQLN1 was detected through RT-PCR. Results: The expression of U2AF65 was suppressed by U2AF65-siRNA vectors. The result of RT-PCR revealed that knockdown... |
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| Keywords: | U2AF65 protein RNA interference UBQLN1 gene alternative splicing |
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