Immunological properties of low molecular-weight proteins binding heavy metals in rat kidney and liver |
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| Authors: | Andrzej J. Żelazowski Jadwiga A. Szymańska Czesklaw S. Cierniewski |
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| Affiliation: | 1. Institute of Environmental Research and Bioanalysis, Medical Academy of Lód?, 90-145 Lód? Poland;2. Institute of Biochemistry and Biophysics, University of Lód?, 90-238 Lód? Poland |
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| Abstract: | Two homogenous fractions of hepatic metallothioneins ((Cd,Zn) MT-1 and (Cd,Zn) MT-2) and renal metal binding proteins ((Bi,Cu) BP-1 and (Bi,Cu) BP-2) were isolated from rats exposed to heavy metals and specific antisera to them were produced in rabbits.These antisera were tested by immunodiffusion and immunoelectrophoresis for their ability to bind different fractions of hepatic Cd,Zn -metallothionein and renal (Bi,Cu)-, (Hg,Cu)- and (Cd,Cu)-binding proteins. It was found that anti (Bi,Cu) BP antisera did not cross-react with hepatic (Cd,Zn) MT-1 and (Cd,Zn) MT-2. Strong immunological cross-reactions were detected between anti (Bi,Cu) BP antisera and individual forms of (Cd,Cu)-, (Hg,Cu)- and (Bi,Cu)-binding proteins isolated from rat kidneys. |
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| Keywords: | (Cd,Zn) MT-1 and 2 fractions 1 and 2 of liver cadmium, zinc-metallothionein (Cd,Cu) BP-1, 2 and 3 fractions 1, 2 and 3 of renal cadmium, copper binding proteins (Hg,Cu) BP-1, 2 and 3 fractions 1, 2 and 3 of renal mercury, copper binding proteins (Bi,Cu) BP-1, 2 and 3 fractions 1, 2 and 3 of renal bismuth, copper binding proteins |
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