The induction of hepatic cytochrome P-450 in C57 BL/10 and DBA/2 mice by isosafrole and piperonyl butoxide. A comparative study with other inducing agents |
| |
| Authors: | T.R Fennell B.C. Sweatman J.W. Bridges |
| |
| Affiliation: | 2. Institute of Industrial and Environmental Health and Safety, and Department of Biochemistry, University of Surrey, Guildford, Surrey, GU2 5XH United Kingdom;1. Department of Drug Metabolism, Wellcome Research Laboratories, Beckenham, Kent United Kingdom |
| |
| Abstract: | Styrene monooxygenase activity was measured in intact nuclear preparations from rat liver by means of a gas chromatographic method. Styrene epoxide formation is NADPH-dependent although it is enhanced when NADH is added with NADPH. This activity is inhibited by microsomal monooxygenase inhibitors SKF 525A and metyrapone and by microsomal epoxide hydrase inhibitors 1,2-epoxy-3,3,3-trichloropropene oxide and cyclohexene oxide. The percentage of inhibition is quantitatively dffferent for the four compounds. Known inducers of liver microsomal monooxygenase show different patterns of induction on nuclear preparations. Phenobarbital induces nuclear monooxygenase activity more than the respective microsomal activity, whereas the contrary holds true for β-naphthoflavone. |
| |
| Keywords: | To whom all correspondence should be addressed. |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
