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The first isolation of two types of trifluoroleucine resistant mutants of Saccharomyces servazzii
Authors:Tatsuya Tominaga  Yohei Okuzawa  Shiro Kato  Motofumi Suzuki
Institution:(1) Saitama Prefectural Industrial Technology Center North Institute, 2-133, Suehiro, Kumagayashi, Saitama, 360-0031, Japan;(2) Cooperative Research Center in Saitama University, 255, Shimookubo, Saitamashi, Saitama, 338-8570, Japan;(3) Japan Collection of Microorganisms, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, 351-0198, Japan
Abstract:Saccharomyces servazzii plays a crucial role in the making of Japanese radish pickles. To make more flavorsome pickles, we sought to generate trifluoroleucine-resistant mutants of S. servazzii. The three resulting mutants could be classified into two types: one that produces more isoamyl alcohol than the parental strain, and one that produces less. The first type has been well documented in Saccharomyces cerevisiae but the latter appears to be novel and has been characterized as such.
Keywords:isoamyl alcohol  pickled Japanese radish  Saccharomyces servazzii  trifluoroleucine
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