| 抗苏云金杆菌毒素Cry1Ac粉纹夜蛾细胞系的特性研究 |
| |
| 引用本文： | 刘凯于 杨红 蒋才富 彭建新 洪华珠. 抗苏云金杆菌毒素Cry1Ac粉纹夜蛾细胞系的特性研究[J]. 实验生物学报, 2004, 37(3): 205-211 |
| |
| 作者姓名： | 刘凯于 杨红 蒋才富 彭建新 洪华珠 |
| |
| 摘 要： | 为了从离体细胞水平探讨昆虫对苏云金芽孢杆菌杀虫晶体蛋白的部分抗性机制，本文采用活化的Cry1AC毒素对粉纹夜蛾BTI-TN-581-4细胞连续筛选86代，获得了高水平抗性细胞，研究了其某些特性。它对Cry1C产生了低水平的交互抗性，对低渗溶液的耐受性显著增强，双向电泳图谱表明抗性细胞膜蛋白组分发生了明显的变化。膜蛋白组分的变化可能导致了筛选细胞的耐低渗透压和抗Cry1C。
|
| 关 键 词： | 粉纹夜蛾细胞系 CrylAc毒素 交互抗性 膜蛋白 苏云金芽孢杆菌 |
Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac] |
| |
| Kai Yu Liu,Hong Yang,Cai Fu Jiang,Jian Xin Peng,Hua Zhu Hong. Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac][J]. Acta Biologiae Experimentalis Sinica, 2004, 37(3): 205-211 |
| |
| Authors: | Kai Yu Liu Hong Yang Cai Fu Jiang Jian Xin Peng Hua Zhu Hong |
| |
| Affiliation: | Institute of Entomology, Central China Normal University, Wuhan 430079, China. |
| |
| Abstract: | The cell line (BTI-TN-5B1-4) of Trichoplusia ni had been selected by Bacilllus thuringiensis (Bt) toxin Cry1Ac for 86 generations continuously. A high level of resistance was achieved and the partial characterizations of the resistance-selected BTI-TN-5B1-4 cells were studied in order to explore the mechanism of the resistance to Bt toxin. The cells selected by Cry1Ac had low level of cross-resistance (19.7 fold) to Cry1C, their ability of tolerance in the hypotonic solution increased evidently (2.5 fold), and there were differential membrane proteins between resistance-selected and unselected susceptible cells on two dimensional electrophoresis maps. We supposed the changes of membrane proteins in resistant cells would be linked to the cross-resistance to Cry1C and the increase of tolerance in hypotonic solution. |
| |
| Keywords: | |
| 本文献已被 维普 等数据库收录！ |
