Generation of superoxide anion by brain endothelial cell xanthine oxidase. |
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| Authors: | L S Terada I R Willingham M E Rosandich J A Leff G W Kindt J E Repine |
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| Institution: | Webb-Waring Lung Institute, Denver, Colorado. |
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| Abstract: | Bovine brain endothelial cells (EC) that were isolated and propagated in pure culture had increased (greater than 20-fold) levels of xanthine oxidase and xanthine dehydrogenase activity compared to whole brain homogenate. Brain EC also released superoxide anion (O2-) into the extracellular medium. Treatment of EC with tungsten decreased (P less than 0.05) both XO activity and O2- release. XO appears to be highly concentrated in cerebral vascular endothelium and may be an important source of O2-. |
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