Isolation and initial characterization of thermotolerant methylotrophic Bacillus strains |
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Authors: | L Dijkhuizen N Arfman MM Attwood AG Brooke W Harder EM Watling |
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Institution: | Department of Microbiology, University of Groningen, Haren, The Netherlands;Department of Microbiology, University of Sheffield, Sheffield, U.K. |
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Abstract: | Abstract Using inocula from a variety of sources, mixed cultures of methanol-utilizing Bacillus strains were enriched readily at 55°C. Isolation of pure cultures was difficult; the six strains that were obtained eventually in pure culture all possessed the RuMP pathway, grew rapidly on methanol at temperatures up to 60° C, and were tolerant to very high methanol concentrations. An NAD-dependent alcohol dehydrogenase appeared to be involved in the initial oxidation of methanol. |
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Keywords: | Methanol Methylotrophic bacilli Thermotolerant methylotrophic bacteria |
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