Characterization of mitochondrial genome of Formosan sambar (Rusa unicolor swinhoei) |
| |
| Authors: | Chia-Hsuan Chen Hsiu-Lin Huang Mu-Tzu Chang Li-Chia Chiang Shih-Lin Cheng Bing-Tsan Liu Chih-Hua Wang Ming-Che Wu and Mu-Chiou Huang |
| |
| Institution: | (1) FRE2584 – CNRS Instabilité du génome et cancer, Pavillon Trouillet-Rossignol, 26 rue d'Ulm, 75248 Paris cedex 05, France;(2) 57 rue Cuvier, 75231 Paris cedex 05, France;(3) CIRAD-EMVT, Programme Ecosystèmes Naturels et Pastoraux, TA 30/F, Campus International de Baillarguet, 34398 Montpellier cedex 5, France;(4) Laboratoire de Génétique Biochimique et Cytogénétique, INRA-CRJ, Domaine de Vilvert, Jouy-en-Josas, 78352 cedex, France |
| |
| Abstract: | The complete mitochondrial genome sequence of the Formosan sambar (Rusa unicolor swinhoei) was obtained by DNA sequencing based on PCR fragments amplified by 26 primer pairs designed by ourselves. The results indicated
that the mtDNA is 16,505 bp in size. This is the first report on mitochondrial DNA (mtDNA) sequence analysis of the Formosan
sambar and the sequence was deposited in the GenBank database under the accession number DQ989636. The complete mitochondrial
sequence included the following gene sequences: 12S and 16S rRNAs, 22 tRNAs and 13 protein-coding genes. The base composition
of the sequence was as follows: A, 33.51%; T, 28.97%; C, 24.07%; and G, 13.46%. The mitochondrial D-loop region was also analyzed
for comparative purposes in the Formosan sambar and 13 other species within the Cervidae family using neighbour-joining method.
The phylogenetic tree demonstrated that there are two separate groups, a European type and an Asian type, within the Cervidae
family. The D-loop sequences of mtDNA of 24 Formosan sambar animals were compared, and the results showed that the Formosan
sambar can be divided into two clades. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
