Metabolism ofl-proline toN-acetyl-d-glucosamine during germ tube formation ofCandida albicans |
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| Authors: | Sachio Hayashi Tamaki Cho Hidenori Kaminishi Dr. Yoshisato Hagihara Kenji Watanabe |
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| Affiliation: | (1) Department of Oral Microbiology, Fukuoka Dental College, 700 Ta, Sawara-ku, 814-01 Fukuoka, Japan;(2) Institute of Microbiology, Faculty of Pharmaceutical Sciences, Fukuoka University, 700 Ta, Sawara-ku, 814-01, Japan |
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| Abstract: | The metabolism ofl-proline toN-acetyl-d-glucosamine (GlcNAc) during germ tube formation ofCandida albicans (C. albicans) ATCC 1002 was studied. In uptake experiments, 6.9 nmol ofl-[14C]proline were taken up by 1×106 cells during 3 h of incubation at 37°C. The percentage of germ tube formation was 94 under the same condition. The presence of GlcNAc reduced the uptake ofl-proline to 3.0 nmol. The percentage of germ tube formation was 95 in the presence and absence of GlcNAc. The [3H]GlcNAc uptake was 3.0 nmol and was constant whetherl-proline was present or not. After the preparation of a chitin fraction from germ tubes that were labeled withl-[14C]proline, the radioactivity froml-proline was detected in the glucosamine (GlcN) fraction by thin-layer chromatography (TLC). The metabolism ofl-proline to GlcNAc in chitin during germ tube formation was confirmed in this experiment. |
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