Evidence for the transit of aminopeptidase N through the basolateral membrane before it reaches the brush border of enterocytes |
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Authors: | Dominique Massey Hélène Feracci Jean-Pierre Gorvel Alain Rigal Jean Michel Soulié Suzanne Maroux |
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Institution: | (1) Centre de Biochimie et de Biologie Moléculaire du CNRS, 13402 Marseille Cedex 9, France |
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Abstract: | Summary
In vivo pulse-chase labeling of rabbit jejunum loops was used in conjunction with subcellular fractionation and quantitative immunoprecipitation to determine whether or not the newly synthesized aminopeptidase N transits through the basolateral membrane before it reaches the apical brush border, its final localization. The kinetics of the arrival of the newly synthesized enzyme in the Golgi complex, basolateral and brush border membrane fractions strongly suggest that on leaving the Golgi aminopeptidase N is transiently integrated into the basolateral domain before reaching the brush border. |
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Keywords: | aminopeptidase brush border cell polarity intracellular transport membrane biogenesis |
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