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Rhipicephalus sanguineus: amino acid composition of egg shell
Authors:B J Jaskoski  V L Butler
Affiliation:Department of Biological Sciences, Loyola University of Chicago, Chicago, Illinois 60626 U.S.A.
Abstract:Acid hydrolysis of the protein fraction of a batch of egg shells of Rhipicephalus sanguineus was followed by determination of the amino acids in the hydrolysate. Using thin layer chromatography, the amino acids—lysine, arginine, aspartic acid, serine, glycine, glutamic acid, alanine, threonine, valine, tyrosine, isoleucine, and leucine were identified. Alkaline hydrolysis of the fraction followed by TLC revealed the presence of tryptophane. Chitin was revealed utilizing a chitosan test.
Keywords:Amino acids   Egg shell   Thin Layer Chromatography   Rhipicephalus sanguineus   Ticks
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