A novel biotransformation of 2-formyl-6-naphthoic acid to 2,6-naphthalene dicarboxylic acid by Pseudomonas sp. for the purification of crude 2,6-naphthalene dicarboxylic acid |
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| Authors: | Dong-Sung Kim Sung-Kyoon Kim Yong-Bok Choi Ik-Hyeon Kwon Kwan-Hwa Park |
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| Affiliation: | (1) R&D Business Labs, Hyosung Corporation, Anyang-Si, Gyeonggi-Do, 431-080, Korea;(2) Department of Food Science and Biotechnology, Seoul National University, Seoul, 151-921, Korea;(3) Department of Food Science and Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul, 151-921, Korea |
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| Abstract: | Crude 2,6-naphthalene dicarboxylic acid was purified by Pseudomonas sp. HN-72 which biotransformed the major impurity of 2-formyl-6-naphthoic acid into 2,6-naphthalene dicarboxylic acid. The biotransformation yield reached 100% when the reaction was performed at 40°C for 1 h, in 200 ml KH2PO4/KOH buffer (50 mM, pH 8.0), with 0.2% (w/v) crude 2,6-naphthalene dicarboxylic acid and 2.5 mg dry cell wt/ml. |
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| Keywords: | Biotransformation Crude 2,6-naphthalene dicarboxylic acid 6-Formyl-2-naphthoic acid Pseudomonas sp. Purification |
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