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Alkaloid production by hairy root cultures in Atropa belladonna |
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| Authors: | Hiroshi Kamada Nobuyuki Okamura Motoyoshi Satake Hiroshi Harada Koichiro Shimomura |
| Institution: | (1) Gene Experiment Center, University of Tsukuba, Sakura-mura, 305 Ibaraki-ken, Japan;(2) Faculty of Pharmaceutical Sciences, Fukuyama University, 985 Higashimura-machi Aza Sanzo, 729-02 Fukuyama-shi, Hiroshima-ken, Japan;(3) Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai Yatabe-machi, Tsukuba-gun, 305 Ibaraki-ken, Japan;(4) Present address: Institute of Biological Sciences, University of Tsukuba, Sakura-mura, 305 Ibaraki-ken, Japan |
| Abstract: | Hairy roots were induced by inoculation of stems of sterile plants of Atropa belladonna with Agrobacterium rhizogenes. The axenic culture of the hairy roots isolated from the stems proliferated 60 fold as based on the initial fresh weight after one month of culture. The presence of atropine and scopolamine in hairy roots were examined by TLC and HPLC. Their amounts were analyzed by GLC. The results show that the amount of the two alkaloids in the axenic cultures was the same as or even higher than those of normal plants grown in the field. |
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