Specific heavy metal labeling of the 3-'terminus of phosphorothioate modified yeast tRNAPhe. |
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Authors: | D J Szalda F Eckstein H Sternbach S J Lippard |
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Institution: | Department of Chemistry, Columbia University and Max-Planck-Institut für Experimentelle Medizin Abteilung Chemie, Göttingen, Germany |
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Abstract: | Yeast tRNAPhe containing a phosphorothioate modified -CS-CS-A terminus binds two moles of chloroterpyridineplatinum(II). This result was determined by titrating the tRNA with 3H](terpy)PtCl] Cl, removing excess platinum by cation exchange chromatography, and determining the amount of bound platinum by radiocounting techniques. It has thus been established that adjacent phosphorothioate modified nucleotides can be labeled with an electron dense stain, a necessary requirement for electronmicroscopic sequencing of polynucleotides to become practical. |
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Keywords: | Address reprint requests to: Dr S J Lippard Department of Chemistry Columbia University New York NY 10027 |
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