| 一株以甘油为底物产二羟丙酮(DHA)微生物菌种的筛选及鉴定 |
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| 引用本文： | 徐美娟,饶志明,沈 微,方慧英,诸葛健.一株以甘油为底物产二羟丙酮(DHA)微生物菌种的筛选及鉴定[J].微生物学报,2008,35(3):0336-0340. |
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| 作者姓名： | 徐美娟 饶志明 沈 微 方慧英 诸葛健 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室 江南大学工业微生物研究中心 无锡 214122;江南大学工业生物技术教育部重点实验室 江南大学工业微生物研究中心 无锡 214122;江南大学工业生物技术教育部重点实验室 江南大学工业微生物研究中心 无锡 214122;江南大学工业生物技术教育部重点实验室 江南大学工业微生物研究中心 无锡 214122;江南大学工业生物技术教育部重点实验室 江南大学工业微生物研究中心 无锡 214122 |
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| 基金项目： | 国家自然科学基金(No. 20676053); 国家“863计划”(No. 2006AA020103); 江苏省青年科技创新人才(学术带头人)基金 (No. BK2006504); 长江学者和创新团队发展计划资助(No. IRT0532) |
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| 摘 要： | 甘油为微生物可利用的理想碳源, 从自然界筛选出21株以甘油为唯一碳源产二羟丙酮(DHA)的菌株, 经初步发酵测定发酵液中DHA含量, 其中菌株6-8 DHA产量最高达6.4 g/L。对其进行常规生理生化鉴定实验, 并结合16S rDNA基因分析, 比对结果表明, 菌株6-8与Acinetobacter sp. 相似性最高, 达99.7%, 在细菌分类学上属于假单胞菌目莫拉菌科不动杆菌属。将其命名为Acinetobacter sp.6-8。
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| 关 键 词： | 甘油 二羟基丙酮 分离筛选 鉴定 |
Screening and Identification of a Strain Producing Dihydroxyacetone During Oxidation of Glycerol |
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| XU Mei-Juan,RAO Zhi-Ming,SHEN Wei,FANG Hui-Ying and ZHUGE Jian.Screening and Identification of a Strain Producing Dihydroxyacetone During Oxidation of Glycerol[J].Acta Microbiologica Sinica,2008,35(3):0336-0340. |
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| Authors: | XU Mei-Juan RAO Zhi-Ming SHEN Wei FANG Hui-Ying and ZHUGE Jian |
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| Institution: | Key laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microorganisms, Jiangnan University, Wuxi 214122;Key laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microorganisms, Jiangnan University, Wuxi 214122;Key laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microorganisms, Jiangnan University, Wuxi 214122;Key laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microorganisms, Jiangnan University, Wuxi 214122;Key laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microorganisms, Jiangnan University, Wuxi 214122 |
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| Abstract: | More than 20 strains capable of producing dihydroxyacetone from glycerol were isolated from 4 different natural environment samples by using two detection methods. The strain 6-8 which could grow on medium containing glycerol as sole carbon source had a higher converting capability. Under a better culture, the highest DHA production of the strain 6-8 reached 6.4 g/L. In addition to general morphological and biochemical characteristics, the strain 6-8 was identified by 16S rDNA sequence and systematic analysis. The results showed that 16S rDNA sequence of the strain 6-8 had similarity of 99.7% with Acinetobacter sp. suggesting that the strain 6-8 is one of subspecies of Acinetobacter sp. |
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| Keywords: | Glycerol Dihydroxyacetone Screening Identification |
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