Phenotypic and genetic features of Escherichia coli strains showing simultaneous expression of localized and diffuse adherence |
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| Authors: | Scaletsky I C Pedroso M Z Silva R M |
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| Affiliation: | 1. Department of Microbiology and Immunology, University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia;2. Burnet Institute, Melbourne, Victoria, Australia;3. Australian Research Council Centre of Excellence in Advanced Molecular Imaging, University of Melbourne, Melbourne, Victoria, Australia;4. Department of Microbiology, Monash University, Australia.;5. Immune therapies Laboratory, Burnet Institute, Melbourne, VIC, Australia,;6. Department of Immunology and Pathology, Central Clinical School, Monash University, Melbourne, VIC, Australia;7. Department of Clinical Pathology, The University of Melbourne, Parkville, VIC, Australia,;8. Department of Epidemiology and Preventive Medicine, Monash University, Melbourne, Victoria, Australia;9. Australian Institute of Tropical Health & Medicine, James Cook University, Cairns, Queensland, Australia;10. Kirby Institute, University of NSW, Sydney, NSW, Australia;11. Department of Microbiology, Royal Melbourne Hospital, Melbourne, Australia;12. WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity;13. Melbourne Sexual Health Centre and Department of Infectious Diseases, Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria, Australia;14. Australian Research Council Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Melbourne, Victoria, Australia |
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| Abstract: | We have previously shown that some Escherichia coli strains isolated from children with diarrhea present the so-called 'localized and diffused adherence (LA/DA) pattern' in which both localized adherence (LA) and diffused adherence (DA) are expressed simultaneously. In the present study, we show that the LA adherence of these strains is genetically and phenotypically similar to that so far described for enteropathogenic E. coli (EPEC) as determined by DNA hybridization and electron microscopy. On the other hand, the DA is encoded by genes not homologous to the DAEC or AIDA-I DNA probes. In addition, the LA/DA strains are able to invade eukaryotic cells both in vitro and in vivo. In the rabbit ileal loop assay their invasion capacity goes beyond the enterocyte and reaches the muscularis mucosae as determined by transmission electron microscopy. These findings suggest that the LA/DA adherence pattern may be linked to a new E. coli virulence category which in the case of the strains studied may be associated to other virulence traits that enable them to more deeply invade the intestinal mucosa. |
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| Keywords: | Diarrhea Escherichia coli Adhesion Localized adherence Diffuse adherence Invasion |
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