Partial Structure, Chromosome Localization, and Expression of the MouseGirk4Gene |
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| Authors: | Kevin Wickman Michael F Seldin Sandra J Gendler David E Clapham |
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| Institution: | aChildren's Hospital Medical Center, Harvard Medical School, Enders Building, Room 1309, 320 Longwood Avenue, Boston, Massachusetts, 02115;bRowe Program in Genetics, Department of Biological Chemistry, The University of California, Davis, 4303 Medical Sciences IA, Davis, California, 95616;cDepartment of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Samuel C. Johnson Research Building, 13400 East Shea Boulevard, Scottsdale, Arizona, 85259 |
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| Abstract: | The G protein-gated potassium channel IKAChconstitutes part of a signaling pathway that mediates the negative chronotropic and inotropic effects of acetylcholine on cardiac physiology. Similar or identical ion channels regulate the excitability of many neurons in response to neurotransmitters. IKAChis composed of two homologous subunits, GIRK1 and GIRK4. Here we describe a partial genomic structure of the mouseGirk4gene. Two exons containing the complete protein-coding sequence were identified.Girk4was mapped to mouse chromosome 9 (13 cM), consistent with the mapping of humanGIRK4to chromosome 11q23–ter. GIRK4 mRNA was found mainly in mouse heart, with trace levels detected in brain, kidney, lung, and spleen. No detectable levels were observed in skeletal muscle, liver, and testis. The onset of GIRK4 mRNA expression in the developing mouse occurs between Embryonic Days 7 and 11, consistent with the appearance and function of the mouse heart. |
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